Bioscience

TGen Researchers Publish New Multiplexed Sequence Approach

September 23, 2008

By Flinn Foundation

[Source: By a GenomeWeb staff reporter , GenomeWeb News] – In a paper appearing online in Nature Methods this weekend, a team of researchers from the Translational Genomics Research Institute described a new multiplexed, targeted re-sequencing strategy for assessing regions of the human genome more quickly and efficiently.

The team used PCR amplicons to create degenerate, indexed DNA barcodes targeting specific regions of the genome before sequencing them with the Illumina Genome Analyzer. The multiplexed approach, they say, is an effective way to uncover genetic variation in genes of interest